QC Analysis

Rna High Sensitivity Tapestation. Appearance of sheared DNA from Agilent 2200 TapeStation analysis Stenglein measured using the Agilent Tapestation High Sensitivity RNA screening tapes and reagents (Agilent, 5067-5579 & 5067-5580). The Agilent 2200 TapeStation system is an automated platform for simpler, faster and more reliable electrophoresis

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A total of 23 RNA samples were used for the benchmark study † Store High Sensitivity RNA Ladder below -20 °C (-4 °F)

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A total of 23 RNA samples were used for the benchmark study 9 For each sample, pipette 1 µL High Sensitivity RNA Sample Buffer ( ) and 2 µL RNA sample in a tube strip or 96-well sample plate1 2 Thaw High Sensitivity RNA Ladder and total RNA samples on ice

Agilent TapeStation BIOSCIENCE CORES. • Scalable throughput, ease of use, constant cost per sample and a fast time to result means that the Agilent 2200 TapeStation system is ideal for RNA quality assessment Both RNA assays are designed to assess the integrity and quantity of total RNA and as such are not intended to analyze DNA samples.

RNA Quality Control using the Agilent 2200 TapeStation System. It is made up of 3 elements: • 2200 TapeStation system (G2964AA) or 2200 TapeStation Nucleic Acid system (G2965AA) † High Sensitivity RNA ScreenTape (5067-5579) with High Sensitivity RNA ScreenTape Sample Buffer (5067-5580) Contaminating intact genomic DNA (20-40kb gDNA) is not detected